Role of lipid peroxidation and PPAR-δ in amplifying glucose-stimulated insulin secretion.

ABSTRACT:

Objective

Previous studies show that polyunsaturated fatty acids (PUFAs) increase the insulin secretory capacity of pancreatic β-cells. We aimed at identifying PUFA-derived mediators and their cellular targets that are involved in the amplification of insulin release from β-cells preexposed to high glucose levels.

Research design and methods

The content of fatty acids in phospholipids of INS-1E β-cells was determined by lipidomics analysis. High-performance liquid chromatography was used to identify peroxidation products in β-cell cultures. Static and dynamic glucose-stimulated insulin secretion (GSIS) assays were performed on isolated rat islets and/or INS-1E cells. The function of peroxisome proliferator-activated receptor-δ (PPAR-δ) in regulating insulin secretion was inv

SUBMITTER: Cohen G 

PROVIDER: S-EPMC3198069 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature