Analysis of H19 methylation in control and abnormal human embryos, sperm and oocytes.

ABSTRACT: ART is suspected to generate increased imprinting errors in the lineage. Following an intra cytoplasmic sperm injection (ICSI) procedure, a certain number of embryos fail to develop normally and imprinting disorders may be associated to the developmental failure. To evaluate this hypothesis, we analysed the methylation profile of H19DMR, a paternally imprinting control region, in high-graded blastocysts, in embryos showing developmental anomalies, in the matching sperm and in oocytes of the concerned couples when they were available. Significant hypomethylation of the paternal allele was observed in half of the embryos, independently of the stage at which they were arrested (morula, compacted morula, pre blastocyst or BC-graded blastocysts). Conversely, some embryos showed significant meth

SUBMITTER: Ibala-Romdhane S 

PROVIDER: S-EPMC3198154 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature