High-resolution imaging reveals indirect coordination of opposite motors and a role for LIS1 in high-load axonal transport.

ABSTRACT: The specific physiological roles of dynein regulatory factors remain poorly understood as a result of their functional complexity and the interdependence of dynein and kinesin motor activities. We used a novel approach to overcome these challenges, combining acute in vivo inhibition with automated high temporal and spatial resolution particle tracking. Acute dynein inhibition in nonneuronal cells caused an immediate dispersal of diverse forms of cargo, resulting from a sharp decrease in microtubule minus-end run length followed by a gradual decrease in plus-end runs. Acute LIS1 inhibition or LIS1 RNA interference had little effect on lysosomes/late endosomes but severely inhibited axonal transport of large, but not small, vesicular structures. Our acute inhibition results argue against dir

SUBMITTER: Yi JY 

PROVIDER: S-EPMC3198168 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature