Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.

ABSTRACT:

Background

Normal development of the atria requires left-right differentiation during embryonic development. Reduced expression of Pitx2c (paired-like homeodomain transcription factor 2, isoform c), a key regulator of left-right asymmetry, has recently been linked to atrial fibrillation. We therefore systematically studied the molecular composition of left and right atrial tissue in adult murine and human atria.

Methods

We compared left and right atrial gene expression in healthy, adult mice of different strains and ages by employing whole genome array analyses on freshly frozen atrial tissue. Selected genes with enriched expression in either atrium were validated by RT-qPCR and Western blot in further animals and in shock-frozen left and right atrial appendages of patients

SUBMITTER: Kahr PC 

PROVIDER: S-EPMC3198471 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature