Structural and functional role of bases 32 and 33 in the anticodon loop of yeast mitochondrial tRNAIle.

ABSTRACT: Previous work has demonstrated the usefulness of the yeast model to investigate the molecular mechanisms underlying defects due to base substitutions in mitochondrial tRNA genes, and to identify suppressing molecules endowed with potential clinical relevance. The present paper extends these investigations to two human equivalent yeast mutations located at positions 32 and 33 in the anticodon loop of tRNA(Ile). Notwithstanding the proximity of the two T>C base substitutions, the effects of these mutations have been found to be quite different in yeast, as they are in human. The T32C substitution has a very severe effect in yeast, consisting in a complete inhibition of growth on nonfermentable substrates. Conversely, respiratory defects caused by the T33C mutation could only be observed in a

SUBMITTER: Montanari A 

PROVIDER: S-EPMC3198592 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature