The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.

ABSTRACT:

Background

Pericentrin (Pcnt), a conserved protein of the pericentriolar material, serves as a multifunctional scaffold for numerous proteins and plays an important role in microtubule organization. Recent studies indicate that Pcnt mutations are associated with a range of diseases including primordial dwarfism and ciliopathies. To date, three Pcnt splice variants from orthologous genes in mice and humans are known.

Principal findings

We generated a specific Pcnt antiserum detecting all known Pcnt splice variants and examined the cellular and subcellular distribution of Pcnt in ciliated tissues of the mouse, the olfactory epithelium and the retina. For the first time, we identified Pcnt and its centrosomal interaction partners at the basal body complex of mouse retinal photo

SUBMITTER: Muhlhans J 

PROVIDER: S-EPMC3198765 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature