Molecular cloning, spatial and temporal expression analysis of CatSper genes in the Chinese Meishan pigs.

ABSTRACT:

Background

Sperm ion channel proteins (CatSpers) are essential for sperm hyperactivated motility, and then penetration through the zona pellucida. The CatSper class of proteins have well been characterized in the mouse and human. However, such data for pigs are not available. In the present study, we cloned the porcine CatSper 1-4 genes, analysed their spatial expression in various organs and temporal expression in the testes from birth until sexual maturity in Meishan boars.

Methods

Rapid amplification of cDNA ends (RACE) was performed to clone the full length cDNAs of porcine CatSper genes and bioinformatics analysis of inferred CatSper proteins was also determined. Various organs were collected from 150 day-old pigs to characterize the spatial expression of CatSper genes

SUBMITTER: Song C 

PROVIDER: S-EPMC3198926 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature