Selection of reference genes for qPCR in hairy root cultures of peanut.

ABSTRACT:

Background

Hairy root cultures produced via Agrobacterium rhizogenes-mediated transformation have emerged as practical biological models to elucidate the biosynthesis of specialized metabolites. To effectively understand the expression patterns of the genes involved in the metabolic pathways of these compounds, reference genes need to be systematically validated under specific experimental conditions as established by the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines. In the present report we describe the first validation of reference genes for RT-qPCR in hairy root cultures of peanut which produce stilbenoids upon elicitor treatments.

Results

A total of 21 candidate reference genes were evaluated. Nineteen genes were selecte

SUBMITTER: Condori J 

PROVIDER: S-EPMC3199266 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature