Characterization of variable EST SSR markers for Norway spruce (Picea abies L.).

ABSTRACT:

Background

Norway spruce is widely distributed across Europe and the predominant tree of the Alpine region. Fast growth and the fact that timber can be harvested cost-effectively in relatively young populations define its status as one of the economically most important tree species of Northern Europe. In this study, EST derived simple sequence repeat (SSR) markers were developed for the assessment of putative functional diversity in Austrian Norway spruce stands.

Results

SSR sequences were identified by analyzing 14,022 publicly available EST sequences. Tri-nucleotide repeat motifs were most abundant in the data set followed by penta- and hexa-nucleotide repeats. Specific primer pairs were designed for sixty loci. Among these, 27 displayed polymorphism in a testing populati

SUBMITTER: Fluch S 

PROVIDER: S-EPMC3199268 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature