Allosteric communication in cysteinyl tRNA synthetase: a network of direct and indirect readout.

ABSTRACT: Protein structure networks are constructed for the identification of long-range signaling pathways in cysteinyl tRNA synthetase (CysRS). Molecular dynamics simulation trajectory of CysRS-ligand complexes were used to determine conformational ensembles in order to gain insight into the allosteric signaling paths. Communication paths between the anticodon binding region and the aminoacylation region have been identified. Extensive interaction between the helix bundle domain and the anticodon binding domain, resulting in structural rigidity in the presence of tRNA, has been detected. Based on the predicted model, six residues along the communication paths have been examined by mutations (single and double) and shown to mediate a coordinated coupling between anticodon recognition and activatio

SUBMITTER: Ghosh A 

PROVIDER: S-EPMC3199515 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature