Analysing GCN4 translational control in yeast by stochastic chemical kinetics modelling and simulation.

ABSTRACT:

Background

The yeast Saccharomyces cerevisiae responds to amino acid starvation by inducing the transcription factor Gcn4. This is mainly mediated via a translational control mechanism dependent upon the translation initiation eIF2·GTP·Met-tRNAiMet ternary complex, and the four short upstream open reading frames (uORFs) in its 5' mRNA leader. These uORFs act to attenuate GCN4 mRNA translation under normal conditions. During amino acid starvation, levels of ternary complex are reduced. This overcomes the GCN4 translation attenuation effect via a scanning/reinitiation control mechanism dependent upon uORF spacing.

Results

Using published experimental data, we have developed and validated a probabilistic formulation of GCN4 translation using the Chemical Master Equation (Model

SUBMITTER: You T 

PROVIDER: S-EPMC3201031 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature