Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.

ABSTRACT: Alternative pre-mRNA splicing allows dramatic expansion of the eukaryotic proteome and facilitates cellular response to changes in environmental conditions. The Saccharomyces cerevisiae gene SUS1, which encodes a protein involved in mRNA export and histone H2B deubiquitination, contains two introns; non-canonical sequences in the first intron contribute to its retention, a common form of alternative splicing in plants and fungi. Here we show that the pattern of SUS1 splicing changes in response to environmental change such as temperature elevation, and the retained intron product is subject to nonsense-mediated decay. The activities of different splicing factors determine the pattern of SUS1 splicing, including intron retention and exon skipping. Unexpectedly, removal of the 3' intron is a

SUBMITTER: Hossain MA 

PROVIDER: S-EPMC3201863 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature