A broad set of different llama antibodies specific for a 16 kDa heat shock protein of Mycobacterium tuberculosis.

ABSTRACT:

Background

Recombinant antibodies are powerful tools in engineering of novel diagnostics. Due to the small size and stable nature of llama antibody domains selected antibodies can serve as a detection reagent in multiplexed and sensitive assays for M. tuberculosis.

Methodology/principal findings

Antibodies for Mycobacterium tuberculosis (M. tb) recognition were raised in Alpaca, and, by phage display, recombinant variable domains of heavy-chain antibodies (VHH) binding to M. tuberculosis antigens were isolated. Two phage display selection strategies were followed: one direct selection using semi-purified protein antigen, and a depletion strategy with lysates, aiming to avoid cross-reaction to other mycobacteria. Both panning methods selected a set of binders with widely diff

SUBMITTER: Trilling AK 

PROVIDER: S-EPMC3202562 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature