A Brassica napus lipase locates at the membrane contact sites involved in chloroplast development.

ABSTRACT:

Background

Fatty acids synthesized in chloroplast are transported to endoplasmic reticulum (ER) for triacylglycerols (TAGs) resembling. The development of chloroplast also requires lipids trafficking from ER to chloroplast. The membrane contact sites (MCSs) between ER and chloroplast has been demonstrated to be involved for the trafficking of lipids and proteins. Lipids trafficking between ER and chloroplast is often accompanied by lipids interconversion. However, it is rarely known how lipids interconversion happens during their trafficking.

Methodology/principal findings

We cloned a lipase gene from Brassica napus L., designated as BnCLIP1. Green fluorescence protein (GFP)-tagged BnCLIP1 was shown to locate at the MCSs between ER and chloroplasts in tobacco leaves. Heterog

SUBMITTER: Tan X 

PROVIDER: S-EPMC3202582 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature