L3MBTL1 deficiency directs the differentiation of human embryonic stem cells toward trophectoderm.

ABSTRACT: Human embryonic stem cells (hESCs) can be used to study the early events in human development and, hopefully, to understand how to differentiate human pluripotent cells for clinical use. To define how L3MBTL1, a chromatin-associated polycomb group protein with transcriptional repressive activities, regulates early events in embryonic cell differentiation, we created hESC lines that constitutively express shRNAs directed against L3MBTL1. The L3MBTL1 knockdown (KD) hESCs maintained normal morphology, proliferation, cell cycle kinetics, cell surface markers, and karyotype after 40 passages. However, under conditions that promote spontaneous differentiation, the L3MBTL1 KD cells differentiated into a relatively homogeneous population of large, flat trophoblast-like cells, unlike the multilinea

SUBMITTER: Hoya-Arias R 

PROVIDER: S-EPMC3202892 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature