Mesenchymal stromal cells from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression.

ABSTRACT: We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characterized the cells, focusing on the issues of gene expression, origin, and cytokine expression. Microarray comparison of early passage neonatal lung MSC gene expression to cord blood MSCs and human fetal and neonatal lung fibroblast lines demonstrated that the neonatal lung MSCs differentially expressed 971 gene probes compared with cord blood MSCs, including the transcription factors Tbx2, Tbx3, Wnt5a, FoxF1, and Gli2, each of which has been associated with lung development. Compa

SUBMITTER: Bozyk PD 

PROVIDER: S-EPMC3202893 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature