Dysregulated selectin expression and monocyte recruitment during ischemia-related vascular remodeling in diabetes mellitus.

ABSTRACT:

Objective

Diabetes mellitus (DM) is associated with impaired ischemia-related vascular remodeling and also dysregulation of the inflammatory response. We sought to determine whether impaired selectin-mediated monocyte recruitment in ischemic tissues contributes to blunted ischemia-mediated angiogenesis in DM.

Methods and results

Contrast-enhanced ultrasound perfusion imaging and molecular imaging of endothelial P-selectin expression in the proximal hindlimb were performed at 1, 3, and 21 days after arterial ligation in wild-type and db/db mice. Ligation reduced muscle blood flow to ≈0.05 mL/minute per gram in both strains. Significant recovery of flow occurred only in wild-type mice (60%-65% of baseline flow). On molecular imaging, baseline P-selectin signal was 4-fold highe

SUBMITTER: Carr CL 

PROVIDER: S-EPMC3202967 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature