A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes.

ABSTRACT:

Background

Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice, protoplasts are commonly prepared from suspension cultured cells or etiolated seedlings, but only a few studies have explored the use of protoplasts from rice green tissue.

Results

Here, we report a simplified method for isolating protoplasts from normally cultivated young rice green tissue without the need for unnecessary chemicals and a vacuum device. Transfections of the generated protoplasts with plasmids of a wide range of sizes (4.5-13

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3203094 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature