Investigation of variation in gene expression profiling of human blood by extended principle component analysis.

ABSTRACT:

Background

Human peripheral blood is a promising material for biomedical research. However, various kinds of biological and technological factors result in a large degree of variation in blood gene expression profiles.

Methodology/principal findings

Human peripheral blood samples were drawn from healthy volunteers and analysed using the Human Genome U133Plus2 Microarray. We applied a novel approach using the Principle Component Analysis and Eigen-R(2) methods to dissect the overall variation of blood gene expression profiles with respect to the interested biological and technological factors. The results indicated that the predominating sources of the variation could be traced to the individual heterogeneity of the relative proportions of different blood cell types (leukocyt

SUBMITTER: Xu Q 

PROVIDER: S-EPMC3203156 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature