Insights into Hox protein function from a large scale combinatorial analysis of protein domains.

ABSTRACT: Protein function is encoded within protein sequence and protein domains. However, how protein domains cooperate within a protein to modulate overall activity and how this impacts functional diversification at the molecular and organism levels remains largely unaddressed. Focusing on three domains of the central class Drosophila Hox transcription factor AbdominalA (AbdA), we used combinatorial domain mutations and most known AbdA developmental functions as biological readouts to investigate how protein domains collectively shape protein activity. The results uncover redundancy, interactivity, and multifunctionality of protein domains as salient features underlying overall AbdA protein activity, providing means to apprehend functional diversity and accounting for the robustness of Hox-contro

SUBMITTER: Merabet S 

PROVIDER: S-EPMC3203194 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature