Comparison of humoral immune responses to Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray.

ABSTRACT:

Background

Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).

Methods

To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.

Results

In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF3

SUBMITTER: Zheng D 

PROVIDER: S-EPMC3203236 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature