How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis.

ABSTRACT:

Background

In order to generate biofuels, insoluble cellulosic substrates are pretreated and subsequently hydrolyzed with cellulases. One way to pretreat cellulose in a safe and environmentally friendly manner is to apply, under mild conditions, non-hydrolyzing proteins such as swollenin - naturally produced in low yields by the fungus Trichoderma reesei. To yield sufficient swollenin for industrial applications, the first aim of this study is to present a new way of producing recombinant swollenin. The main objective is to show how swollenin quantitatively affects relevant physical properties of cellulosic substrates and how it affects subsequent hydrolysis.

Results

After expression in the yeast Kluyveromyces lactis, the resulting swollenin was purified. The adsorption para

SUBMITTER: Jager G 

PROVIDER: S-EPMC3203333 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature