Viral kinetics of enterovirus 71 in human abdomyosarcoma cells.

ABSTRACT:

Aim

To characterise the viral kinetics of enterovirus 71 (EV71).

Methods

In this study, human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI). After infection, the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD camera at different time points post viral infection (0, 6, 12, 24 h post infection). Cell growth and viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in both EV71 infected and mock infected cells at each time point. EV71 replication kinetics in RD cells was determined by measuring the total intracellular viral RNA with real-time reverse-transcription polymerase chain reaction (qRT-PCR). Also, the intracellular

SUBMITTER: Lu J 

PROVIDER: S-EPMC3203367 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature