Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.

ABSTRACT: Many genes have several, sometimes divergent functions during development. Therefore, timing of gene knockdown for functional analysis during development has to be done with precise temporal control, as loss of a gene's function at early stages prevents its analysis later in development. RNAi, in combination with the accessibility of chicken embryos, is an effective approach for temporally controlled analysis of gene function during neural development. Here, we describe novel plasmid vectors that contain cell type-specific promoters/enhancers to drive the expression of a fluorescent marker, followed directly by a miR30-RNAi transcript for gene silencing. These vectors allow for direct tracing of cells experiencing gene silencing by the bright fluorescence. The level of knockdown is suffici

SUBMITTER: Wilson NH 

PROVIDER: S-EPMC3203593 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature