Evolutionary genome engineering using a restriction-modification system.

ABSTRACT: Modification of complex microbial cellular processes is often necessary to obtain organisms with particularly favorable characteristics, but such experiments can take many generations to achieve. In the present article, we accelerated the experimental evolution of Escherichia coli populations under selection for improved growth using one of the restriction-modification systems, which have shaped bacterial genomes. This resulted in faster evolutionary changes in both the genome and bacterial growth. Transcriptome/genome analysis at various stages enabled prompt identification of sequential genome rearrangements and dynamic gene-expression changes associated with growth improvement. The changes were related to cell-to-cell communication, the cell death program, as well as mass production and

SUBMITTER: Asakura Y 

PROVIDER: S-EPMC3203608 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature