Conformational change of single-stranded RNAs induced by liposome binding.

ABSTRACT: The interaction between single-stranded RNAs and liposomes was studied using UV, Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD). The effect of the surface characteristics of liposomes, which were composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and modified with cholesterol (Ch) or 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), on the liposome-RNA interaction was investigated. The fluorescence of 6-(p-toluidino)naphthalene-2-sulfonate (TNS) embedded in the liposome surface (ε = 30-40) was decreased in the presence of tRNA, suggesting that single-stranded tRNA could bind onto the liposome. The dehydration of -PO₂⁻-, guanine (G) and cytosine (C) of tRNA molecules in the presence of liposomes suggested both an electrostatic intera

SUBMITTER: Suga K 

PROVIDER: S-EPMC3203612 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature