IgA1 immune complexes from pediatric patients with IgA nephropathy activate cultured human mesangial cells.

ABSTRACT:

Background

Circulating immune complexes (CIC) containing galactose (Gal)-deficient IgA1 from adults with IgA nephropathy (IgAN) induce proliferation of cultured mesangial cells, but activities of CIC from pediatric patients with the disease have not been studied.

Methods

CIC of different sizes were isolated from sera of pediatric and adult IgAN patients and their effects on cultured human mesangial cells (MC) were assessed by measuring cellular proliferation, expression of IL-6 and IL-8 and laminin and phosphotyrosine signaling.

Results

Large CIC from pediatric IgAN patients (>800 kDa) containing Gal-deficient IgA1 stimulated cellular proliferation, whereas in some patients, smaller CIC were inhibitory. Addition of stimulatory and inhibitory CIC to MC differentially a

SUBMITTER: Novak J 

PROVIDER: S-EPMC3203630 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature