Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus.

ABSTRACT: Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere to mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain to be elucidated for many prebiotic substrates. Although β-galactooligosaccharides (GOS) are documented prebiotic compounds, little is known about their utilization by lactobacilli. This study aimed to identify genetic loci in Lactobacillus acidophilus NCFM responsible for the transport and catabolism of GOS. Whole-genome oligonucleotide microarrays were used to survey the differential global transcriptome during logarithmic growth of L. acidophilus NCFM using GOS or glucose as a sole sou

SUBMITTER: Andersen JM 

PROVIDER: S-EPMC3203779 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature