Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation.

ABSTRACT: Autophagy is an evolutionarily conserved pathway for the degradation of intracellular contents. How autophagy is regulated, especially upon changes in metabolic and nutritional state, remains poorly understood. By using a prototrophic strain of Saccharomyces cerevisiae, we observed that, unexpectedly, autophagy is strongly induced simply upon switch from a rich medium to a minimal medium in the complete absence of nitrogen starvation. This novel form of autophagy was termed "non-nitrogen-starvation (NNS)-induced autophagy." A visual screen uncovered three regulators of autophagy-Iml1p, Npr2p, and Npr3p-which function in the same complex and are selectively required for NNS-induced autophagy. During NNS-induced autophagy, Iml1p localized to either preautophagosomal structures (PAS) or non-P

SUBMITTER: Wu X 

PROVIDER: S-EPMC3204073 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature