Dataset Information

Oseltamivir-resistant pandemic (H1N1) 2009 virus, Mexico.

ABSTRACT: During May 2009-April 2010, we analyzed 692 samples of pandemic (H1N1) 2009 virus from patients in Mexico. We detected the H275Y substitution of the neuraminidase gene in a specimen from an infant with pandemic (H1N1) 2009 who was treated with oseltamivir. This virus was susceptible to zanamivir and resistant to adamantanes and oseltamivir.

SUBMITTER: Ramirez-Gonzalez JE

PROVIDER: S-EPMC3204764 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Oseltamivir-resistant pandemic (H1N1) 2009 virus, Mexico.

Ramirez-Gonzalez José Ernesto JE Gonzalez-Duran Elizabeth E Alcantara-Perez Patricia P Wong-Arambula Claudia C Olivera-Diaz Hiram H Cortez-Ortiz Iliana I Barrera-Badillo Gisela G Nguyen Ha H Gubareva Larisa L Lopez-Martinez Irma I Díaz-Quiñonez Jose Alberto JA Lezana-Fernández Miguel Angel MA Gatell-Ramírez Hugo Lopez HL Cordova Villalobos Jose Angel JA Hernández-Avila Mauricio M Alpuche-Aranda Celia C

Emerging infectious diseases 20110201 2

During May 2009-April 2010, we analyzed 692 samples of pandemic (H1N1) 2009 virus from patients in Mexico. We detected the H275Y substitution of the neuraminidase gene in a specimen from an infant with pandemic (H1N1) 2009 who was treated with oseltamivir. This virus was susceptible to zanamivir and resistant to adamantanes and oseltamivir. ...[more]

PMID: 21291607

Similar Datasets

Project description:The neuraminidase (NA) inhibitor oseltamivir offers an important immediate option for the control of influenza, and its clinical use has increased substantially during the recent H1N1 pandemic. In view of the high prevalence of oseltamivir-resistant seasonal H1N1 influenza viruses in 2007-2008, there is an urgent need to characterize the transmissibility and fitness of oseltamivir-resistant H1N1/2009 viruses, although resistant variants have been isolated at a low rate. Here we studied the transmissibility of a closely matched pair of pandemic H1N1/2009 clinical isolates, one oseltamivir-sensitive and one resistant, in the ferret model. The resistant H275Y mutant was derived from a patient on oseltamivir prophylaxis and was the first oseltamivir-resistant isolate of the pandemic virus. Full genome sequencing revealed that the pair of viruses differed only at NA amino acid position 275. We found that the oseltamivir-resistant H1N1/2009 virus was not transmitted efficiently in ferrets via respiratory droplets (0/2), while it retained efficient transmission via direct contact (2/2). The sensitive H1N1/2009 virus was efficiently transmitted via both routes (2/2 and 1/2, respectively). The wild-type H1N1/2009 and the resistant mutant appeared to cause a similar disease course in ferrets without apparent attenuation of clinical signs. We compared viral fitness within the host by co-infecting a ferret with oseltamivir-sensitive and -resistant H1N1/2009 viruses and found that the resistant virus showed less growth capability (fitness). The NA of the resistant virus showed reduced substrate-binding affinity and catalytic activity in vitro and delayed initial growth in MDCK and MDCK-SIAT1 cells. These findings may in part explain its less efficient transmission. The fact that the oseltamivir-resistant H1N1/2009 virus retained efficient transmission through direct contact underlines the necessity of continuous monitoring of drug resistance and characterization of possible evolving viral proteins during the pandemic.

Dataset Information

Oseltamivir-resistant pandemic (H1N1) 2009 virus, Mexico.

Publications

Oseltamivir-resistant pandemic (H1N1) 2009 virus, Mexico.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets