Slingshot isoform-specific regulation of cofilin-mediated vascular smooth muscle cell migration and neointima formation.

ABSTRACT:

Objective

We hypothesized that cofilin activation by members of the slingshot (SSH) phosphatase family is a key mechanism regulating vascular smooth muscle cell (VSMC) migration and neoinitima formation following vascular injury.

Methods and results

Scratch wound and modified Boyden chamber assays were used to assess VSMC migration following downregulation of the expression of cofilin and each SSH phosphatase isoform (SSH1, SSH2, and SSH3) by small interfering RNA (siRNA), respectively. Cofilin siRNA greatly attenuated the ability of VSMC migration into the "wound," and platelet-derived growth factor (PDGF)-induced migration was virtually eliminated versus a 3.5-fold increase in nontreated VSMCs, establishing a critical role for cofilin in VSMC migration. Cofilin activation

SUBMITTER: Torres RA 

PROVIDER: S-EPMC3204790 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature