Differential gene expression at coral settlement and metamorphosis--a subtractive hybridization study.

ABSTRACT:

Background

A successful metamorphosis from a planktonic larva to a settled polyp, which under favorable conditions will establish a future colony, is critical for the survival of corals. However, in contrast to the situation in other animals, e.g., frogs and insects, little is known about the molecular basis of coral metamorphosis. We have begun to redress this situation with previous microarray studies, but there is still a great deal to learn. In the present paper we have utilized a different technology, subtractive hybridization, to characterize genes differentially expressed across this developmental transition and to compare the success of this method to microarray.

Methodology/principal findings

Suppressive subtractive hybridization (SSH) was used to identify two pools

SUBMITTER: Hayward DC 

PROVIDER: S-EPMC3204972 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature