A simple magnetic separation method for high-yield isolation of pure primary microglia.

ABSTRACT: Microglial cells play a dynamic role in the brain beyond their established function of immune surveillance. Activated microglia play key roles in neural development, neuroinflammation, neural repair and neurotoxicity. They are particularly important in several neurodegenerative diseases in which sustained microglial activation contributes to the progression of neurodegenerative processes. Consequently, understanding microglial function in CNS health and disease has become an area of active research in recent years. However, a significant obstacle to progress in this field has been the inherent difficulties in obtaining large amounts of primary microglial cells to routinely perform mechanistic studies and characterize signaling pathways regulating the dynamics of microglial activation. Here

SUBMITTER: Gordon R 

PROVIDER: S-EPMC3205446 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature