Genome-wide depletion of replication initiation events in highly transcribed regions.

ABSTRACT: This report investigates the mechanisms by which mammalian cells coordinate DNA replication with transcription and chromatin assembly. In yeast, DNA replication initiates within nucleosome-free regions, but studies in mammalian cells have not revealed a similar relationship. Here, we have used genome-wide massively parallel sequencing to map replication initiation events, thereby creating a database of all replication initiation sites within nonrepetitive DNA in two human cell lines. Mining this database revealed that genomic regions transcribed at moderate levels were generally associated with high replication initiation frequency. In genomic regions with high rates of transcription, very few replication initiation events were detected. High-resolution mapping of replication initiation si

SUBMITTER: Martin MM 

PROVIDER: S-EPMC3205567 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature