Genotypic characterization of Cryptosporidium hominis from water samples in Sao Paulo, Brazil.

ABSTRACT: The protozoan parasite Cryptosporidium has emerged as one of the most important water contaminants, causing waterborne outbreaks of diarrheal diseases worldwide. The small size of oocysts under the microscope and the possibility of changes in characteristics of oocysts, mainly in environmental samples, make the taxonomy of the genus difficult if morphologic characteristics are considered. This limitation encouraged the application of molecular methods to identify this microorganism. The aim of this study was to detect and identify by nested-polymerase chain reaction oocysts of Cryptosporidium present in water samples in the state of São Paulo, Brazil. Water samples were concentrated through a membrane filter, DNA was extracted by using a standard technique, and both amplification reactions

SUBMITTER: Araujo RS 

PROVIDER: S-EPMC3205628 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature