Unique gene expression profile associated with an early-onset multiple endocrine neoplasia (MEN1)-associated pituitary adenoma.

ABSTRACT:

Context

Multiple endocrine neoplasia type 1 (MEN1) is caused by mutations in the menin (MEN1) gene. The mechanism(s) by which MEN1 mutations lead to pituitary tumor formation remain(s) unknown.

Objective

The aim of the study was to identify the pediatric MEN1-associated pituitary tumor transcriptome.

Patients and methods

A patient harboring a MEN1 mutation (c.525C>G; p.H139D) who presented with an early-onset mammosomatotroph pituitary adenoma was studied. Microarray analysis was performed in the tumor sample and compared with the profile observed in normal pituitaries and in a sporadic mammosomatotropinoma. Validation of the microarray results was performed using quantitative real-time PCR and immunohistochemical analysis for selected genes.

Results

In the MEN

SUBMITTER: Farrell WE 

PROVIDER: S-EPMC3205896 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature