Silencing of p130cas in ovarian carcinoma: a novel mechanism for tumor cell death.

ABSTRACT:

Background

We investigated the clinical and biological significance of p130cas, an important cell signaling molecule, in ovarian carcinoma.

Methods

Expression of p130cas in ovarian tumors, as assessed by immunohistochemistry, was associated with tumor characteristics and patient survival. The effects of p130cas gene silencing with small interfering RNAs incorporated into neutral nanoliposomes (siRNA-DOPC), alone and in combination with docetaxel, on in vivo tumor growth and on tumor cell proliferation (proliferating cell nuclear antigen) and apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling) were examined in mice bearing orthotopic taxane-sensitive (HeyA8 and SKOV3ip1) or taxane-resistant (HeyA8-MDR) ovarian tumors (n = 10 per group). To determine the s

SUBMITTER: Nick AM 

PROVIDER: S-EPMC3206039 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature