Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas).

ABSTRACT:

Background

Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining.

Results

In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by

SUBMITTER: Wang Z 

PROVIDER: S-EPMC3206431 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature