Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions.

ABSTRACT:

Background

The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation methods have been used to study plant-pathogen interactions in this model system. However, none of the methods reported to date are similar to those occurring in nature and amicable to large-scale mutant screens.

Results

In this study, we developed a rapid and reliable seedling flood-inoculation method based on young Arabidopsis seedlings grown on MS medium. This method has several advantages over conventional soil-grown plant inoculation assays, including a shorter growth and incubation period, ease of inoculation and handling, uniform infection and disease deve

SUBMITTER: Ishiga Y 

PROVIDER: S-EPMC3206466 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature