A fitness assay for comparing RNAi effects across multiple C. elegans genotypes.

ABSTRACT:

Background

RNAi technology by feeding of E. coli containing dsRNA in C. elegans has significantly contributed to further our understanding of many different fields, including genetics, molecular biology, developmental biology and functional genomics. Most of this research has been carried out in a single genotype or genetic background. However, RNAi effects in one genotype do not reveal the allelic effects that segregate in natural populations and contribute to phenotypic variation.

Results

Here we present a method that allows for rapidly comparing RNAi effects among diverse genotypes at an improved high throughput rate. It is based on assessing the fitness of a population of worms by measuring the rate at which E. coli is consumed. Critically, we demonstrate the analytical

SUBMITTER: Elvin M 

PROVIDER: S-EPMC3206879 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature