A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling.

ABSTRACT: To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed N. attenuata. Our results are consistent with the source-to-sink movement of an sRNA silencing signal.

SUBMITTER: Fragoso V 

PROVIDER: S-EPMC3207920 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature