Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by β(1)-integrins.

ABSTRACT: The hypothesis tested by these studies states that in addition to interendothelial cell tight junction proteins, matrix adhesion by β(1)-integrin receptors expressed by endothelial cells have an important role in maintaining the cerebral microvessel permeability barrier. Primary brain endothelial cells from C57 BL/6 mice were incubated with β(1)-integrin function-blocking antibody (Ha2/5) or isotype control and the impacts on claudin-5 expression and microvessel permeability were quantified. Both flow cytometry and immunofluorescence studies demonstrated that the interendothelial claudin-5 expression by confluent endothelial cells was significantly decreased in a time-dependent manner by Ha2/5 exposure relative to isotype. Furthermore, to assess the barrier properties, transendothelial ele

SUBMITTER: Osada T 

PROVIDER: S-EPMC3208159 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature