NeuriteQuant: an open source toolkit for high content screens of neuronal morphogenesis.

ABSTRACT:

Background

To date, some of the most useful and physiologically relevant neuronal cell culture systems, such as high density co-cultures of astrocytes and primary hippocampal neurons, or differentiated stem cell-derived cultures, are characterized by high cell density and partially overlapping cellular structures. Efficient analytical strategies are required to enable rapid, reliable, quantitative analysis of neuronal morphology in these valuable model systems.

Results

Here we present the development and validation of a novel bioinformatics pipeline called NeuriteQuant. This tool enables fully automated morphological analysis of large-scale image data from neuronal cultures or brain sections that display a high degree of complexity and overlap of neuronal outgrowths. It also

SUBMITTER: Dehmelt L 

PROVIDER: S-EPMC3208608 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature