Multicopy suppression screening of Saccharomyces cerevisiae Identifies the ubiquitination machinery as a main target for improving growth at low temperatures.

ABSTRACT: A decrease in ambient temperature alters membrane functionality and impairs the proper interaction between the cell and its external milieu. Understanding how cells adapt membrane properties and modulate the activity of membrane-associated proteins is therefore of major interest from both the basic and the applied points of view. Here, we have isolated multicopy suppressors of the cold sensitivity phenotype of a trp1 strain of Saccharomyces cerevisiae. Three poorly characterized genes, namely, ALY2 encoding the endocytic adaptor, CAJ1 encoding the J protein, and UBP13 encoding the ubiquitin C-terminal hydrolase, were identified as mediating increased growth at 12°C of both Trp⁻ and Trp+ yeast strains. This effect was likely due to the downregulation of cold-instigated degradation of nutrie

SUBMITTER: Hernandez-Lopez MJ 

PROVIDER: S-EPMC3209179 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature