Regulation of perR expression by iron and PerR in Campylobacter jejuni.

ABSTRACT: Campylobacter jejuni is a leading food-borne pathogen causing gastroenteritis in humans. Although OxyR is a widespread oxidative stress regulator in many Gram-negative bacteria, C. jejuni lacks OxyR and instead possesses the metalloregulator PerR. Despite the important role played by PerR in oxidative stress defense, little is known about the factors influencing perR expression in C. jejuni. In this study, a perR promoter-lacZ fusion assay demonstrated that iron significantly reduced the level of perR transcription, whereas other metal ions, such as copper, cobalt, manganese, and zinc, did not affect perR transcription. Notably, a perR mutation substantially increased the level of perR transcription and in trans complementation restored the transcriptional changes, suggesting perR is trans

SUBMITTER: Kim M 

PROVIDER: S-EPMC3209207 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature