The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach.

ABSTRACT:

Background

Cellular function is regulated by the balance of stringently regulated amounts of mRNA. Previous reports revealed that RNA polymerase II (RNAPII), which transcribes mRNA, can be classified into the pausing state and the active transcription state according to the phosphorylation state of RPB1, the catalytic subunit of RNAPII. However, genome-wide association between mRNA expression level and the phosphorylation state of RNAPII is unclear. While the functional importance of pausing genes is clear, such as in mouse Embryonic Stem cells for differentiation, understanding this association is critical for distinguishing pausing genes from active transcribing genes in expression profiling data, such as microarrays and RNAseq. Therefore, we examined the correlation between the

SUBMITTER: Odawara J 

PROVIDER: S-EPMC3209707 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature