Rapidly optimizing an aptamer based BoNT sensor by feedback system control (FSC) scheme.

ABSTRACT: The sensitivity and detection time of an aptamer based biosensor for detecting botulinum neurotoxin (BoNT) depend upon the formation of proper tertiary architecture of aptamer, which closely correlates with the combinatorial effects of multiple types of ions and their concentrations presented in the buffer. Finding the optimal conditions for four different ions at 12 different concentrations, 20,736 possible combinations, by brute force is an extremely laborious and time-consuming task. Here, we introduce a feedback system control (FSC) scheme that can rapidly identify the best combination of components to form the optimal aptamer structure binding to a target molecule. In this study, rapid identification of optimized ionic combinations for electrochemical aptasensor of BoNT type A (BoNT/A

SUBMITTER: Wei F 

PROVIDER: S-EPMC3210382 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature