Mitral valve endothelial cells with osteogenic differentiation potential.

ABSTRACT:

Objective

Cardiac valvular endothelium is unique in its ability to undergo endothelial-to-mesenchymal transformation, a differentiation process that is essential for valve development and has been proposed as mechanism for replenishing the interstitial cells of mature valves. We hypothesized that the valvular endothelium contains endothelial cells that are direct precursors to osteoblastic valvular interstitial cells (VICs).

Methods and results

Clonal cell populations from ovine mitral valve leaflets were isolated by single cell plating. Mitral valvular endothelial and mesenchymal clones were tested for osteogenic, adipogenic, and chondrogenic differentiation, determined by the expression of lineage-specific markers. Mitral valvular endothelial clones showed a propensity for

SUBMITTER: Wylie-Sears J 

PROVIDER: S-EPMC3210435 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature