Tumor engraftment in nude mice and enrichment in stroma- related gene pathways predict poor survival and resistance to gemcitabine in patients with pancreatic cancer.

ABSTRACT:

Purpose

The goal of this study was to evaluate prospectively the engraftment rate, factors influencing engraftment, and predictability of clinical outcome of low-passage xenografts from patients with resectable pancreatic ductal adenocarcinoma (PDA) and to establish a bank of PDA xenografts.

Experimental design

Patients with resectable PDA scheduled for resection at the Johns Hopkins Hospital were eligible. Representative pieces of tumor were implanted in nude mice. The status of the SMAD4 gene and content of tumor-generating cells were determined by immunohistochemistry. Gene expression was carried out by using a U133 Plus 2.0 array. Patients were followed for progression and survival.

Results

A total of 94 patients with PDA were resected, 69 tumors implanted in nude

SUBMITTER: Garrido-Laguna I 

PROVIDER: S-EPMC3210576 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature