Gene expression variations in microsatellite stable and unstable colon cancer cells.

ABSTRACT:

Background

Microsatellite instability (MSI) is a marker of chemoresistance, but it is associated with improved survival compared with microsatellite-stable (MSS) colon cancers. We hypothesized that MSI tumors overexpress chemoresistance-associated genes and underexpress DNA damage/repair genes. We used ultra high-throughput sequencing (UHTS) to assess the expression of representative genes in MSI and MSS colon cancer cell lines.

Methods

Solexa UHTS was used to examine gene expression in HCT116 (MSI) and HT29 (MSS) cells, and normal colonic mucosa (NCM). We compared expression of 40 genes involved in chemoresistance, DNA repair, DNA damage, and drug metabolism pathways.

Results

We observed gene expression differences between MSI and MSS cell lines in 8 out of 40 genes

SUBMITTER: Duldulao MP 

PROVIDER: S-EPMC3210903 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature